pdh e1α (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology
pdh e1α
Pdh E1α, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 128 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pdh+e1%CE%B1/PDH-E1%CE%B1+Antibody/10__3390_slash_antiox15040410-71-66-74
Average 94 stars, based on 128 article reviews
Pdh E1α, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 128 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pdh+e1%CE%B1/PDH-E1%CE%B1+Antibody/10__3390_slash_antiox15040410-71-66-74
Average 94 stars, based on 128 article reviews
pdh e1α - by Bioz Stars,
2026-10
94/100 stars
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Incubation:Article Title: Mitochondrial Functionality Is Regulated by Alkylphospholipids in Human Colon Cancer Cells Article Snippet: .. Then, proteins were electrotransferred onto nitrocellulose membranes with the Trans-blot Turbo system (Bio-Rad) and incubated in a blocking solution of 5% non-fat powdered milk in TBS-Tween (0.05% Tween 20) for 1 h. The following antibodies were incubated overnight at 4 °C: total OXPHOS human WB antibody cocktail (1:500 dilution, #MS601, Abcam, Orlando, FL, USA); PARP (1:1000 dilution, 46D11, #9542, Cell Signaling Technology, Danvers, MA, USA); IDH2 (1:1000 dilution, D7H6Q, #12652, Cell Signaling Technology, Danvers, MA, USA); LC3A/B (1:1000 dilution, #4108, Cell Signaling Technology, Danvers, MA, USA); UCP2 (1:1000 dilution, G-6, #sc-390189, Santa Cruz Biotechnology, Santa Cruz, CA, USA); Article Title: A tryptophan-derived uremic metabolite/Ahr/Pdk4 axis governs skeletal muscle mitochondrial energetics in chronic kidney disease Article Snippet: .. The membranes were then incubated for 2 hours in blocking solution with secondary antibody (Licor, C80118-05, 1:10,000 dilution) to detect the AHR, Article Title: Mitochondrial Functionality Is Regulated by Alkylphospholipids in Human Colon Cancer Cells. Article Snippet: .. Then, proteins were electrotransferred onto nitrocellulose membranes with the Trans-blot Turbo system (Bio-Rad) and incubated in a blocking solution of 5% non-fat powdered milk in TBS-Tween (0.05% Tween 20) for 1 h. The following antibodies were incubated overnight at 4 ◦C: total OXPHOS human WB antibody cocktail (1:500 dilution, #MS601, Abcam, Orlando, FL, USA); PARP (1:1000 dilution, 46D11, #9542, Cell Signaling Technology, Danvers, MA, USA); IDH2 (1:1000 dilution, D7H6Q, #12652, Cell Signaling Technology, Danvers, MA, USA); LC3A/B (1:1000 dilution, #4108, Cell Signaling Technology, Danvers, MA, USA); UCP2 (1:1000 dilution, G-6, #sc-390189, Santa Cruz Biotechnology, Santa Cruz, CA, USA); Article Title: Oleocanthal Induces Mitochondrial Dysfunction in Breast Cancer Cell Lines Depending on c-MET Expression Article Snippet: For Western Blots, 20 μg of protein were separated into SDS-PAGE gels and were electrotransferred onto nitrocellulose membranes with the Trans-blot® TurboTM Transfer System from Bio-Rad (Hercules, CA, USA). .. Membranes were blocked using a solution of 5% (w/v) non-fat powdered milk in TBS with 0.05% (v/v) Tween, for 1 h. The membranes were incubated overnight at 4 ◦C with the following antibodies: total OXPHOS antibody cocktail (ab110411, 1:1000) from Abcam (Orlando, FL, USA); LDHA (2012, 1:1000) and IDH2 (12652, 1:1000) from Cell Signaling Technology (Danvers, MA, USA); CS (1631-1-AP, 1:8000) from Proteintech (Rosemont, IL, USA); Blocking Assay:Article Title: Mitochondrial Functionality Is Regulated by Alkylphospholipids in Human Colon Cancer Cells Article Snippet: .. Then, proteins were electrotransferred onto nitrocellulose membranes with the Trans-blot Turbo system (Bio-Rad) and incubated in a blocking solution of 5% non-fat powdered milk in TBS-Tween (0.05% Tween 20) for 1 h. The following antibodies were incubated overnight at 4 °C: total OXPHOS human WB antibody cocktail (1:500 dilution, #MS601, Abcam, Orlando, FL, USA); PARP (1:1000 dilution, 46D11, #9542, Cell Signaling Technology, Danvers, MA, USA); IDH2 (1:1000 dilution, D7H6Q, #12652, Cell Signaling Technology, Danvers, MA, USA); LC3A/B (1:1000 dilution, #4108, Cell Signaling Technology, Danvers, MA, USA); UCP2 (1:1000 dilution, G-6, #sc-390189, Santa Cruz Biotechnology, Santa Cruz, CA, USA); Article Title: A tryptophan-derived uremic metabolite/Ahr/Pdk4 axis governs skeletal muscle mitochondrial energetics in chronic kidney disease Article Snippet: .. The membranes were then incubated for 2 hours in blocking solution with secondary antibody (Licor, C80118-05, 1:10,000 dilution) to detect the AHR, Article Title: Mitochondrial Function Differences between Tumor Tissue of Human Metastatic and Premetastatic CRC Article Snippet: .. Following 1h of blocking with 5% non-fat powdered milk, primary antibodies were added against the OXPHOS complexes (Abcam, Bristol, UK; Cat# ab110411, RRID:AB_2756818); SIRT1 (Cell Signaling Technology, Danvers, MA; Cat# 8469, RRID:AB_10999470); PGC1α (Abcam Cat# ab54481, RRID:AB_881987); TFAM (Cell Signaling Technology, Danvers, MA, USA; Cat# 7495, RRID:AB_10841294); LDHA (Cell Signaling Technology, Danvers, MA, USA; Cat# 2012, RRID:AB_2137173); Article Title: Mitochondrial Functionality Is Regulated by Alkylphospholipids in Human Colon Cancer Cells. Article Snippet: .. Then, proteins were electrotransferred onto nitrocellulose membranes with the Trans-blot Turbo system (Bio-Rad) and incubated in a blocking solution of 5% non-fat powdered milk in TBS-Tween (0.05% Tween 20) for 1 h. The following antibodies were incubated overnight at 4 ◦C: total OXPHOS human WB antibody cocktail (1:500 dilution, #MS601, Abcam, Orlando, FL, USA); PARP (1:1000 dilution, 46D11, #9542, Cell Signaling Technology, Danvers, MA, USA); IDH2 (1:1000 dilution, D7H6Q, #12652, Cell Signaling Technology, Danvers, MA, USA); LC3A/B (1:1000 dilution, #4108, Cell Signaling Technology, Danvers, MA, USA); UCP2 (1:1000 dilution, G-6, #sc-390189, Santa Cruz Biotechnology, Santa Cruz, CA, USA); Western Blot:Article Title: Mitochondrial Functionality Is Regulated by Alkylphospholipids in Human Colon Cancer Cells Article Snippet: .. Then, proteins were electrotransferred onto nitrocellulose membranes with the Trans-blot Turbo system (Bio-Rad) and incubated in a blocking solution of 5% non-fat powdered milk in TBS-Tween (0.05% Tween 20) for 1 h. The following antibodies were incubated overnight at 4 °C: total OXPHOS human WB antibody cocktail (1:500 dilution, #MS601, Abcam, Orlando, FL, USA); PARP (1:1000 dilution, 46D11, #9542, Cell Signaling Technology, Danvers, MA, USA); IDH2 (1:1000 dilution, D7H6Q, #12652, Cell Signaling Technology, Danvers, MA, USA); LC3A/B (1:1000 dilution, #4108, Cell Signaling Technology, Danvers, MA, USA); UCP2 (1:1000 dilution, G-6, #sc-390189, Santa Cruz Biotechnology, Santa Cruz, CA, USA); Article Title: Mitochondrial Functionality Is Regulated by Alkylphospholipids in Human Colon Cancer Cells. Article Snippet: .. Then, proteins were electrotransferred onto nitrocellulose membranes with the Trans-blot Turbo system (Bio-Rad) and incubated in a blocking solution of 5% non-fat powdered milk in TBS-Tween (0.05% Tween 20) for 1 h. The following antibodies were incubated overnight at 4 ◦C: total OXPHOS human WB antibody cocktail (1:500 dilution, #MS601, Abcam, Orlando, FL, USA); PARP (1:1000 dilution, 46D11, #9542, Cell Signaling Technology, Danvers, MA, USA); IDH2 (1:1000 dilution, D7H6Q, #12652, Cell Signaling Technology, Danvers, MA, USA); LC3A/B (1:1000 dilution, #4108, Cell Signaling Technology, Danvers, MA, USA); UCP2 (1:1000 dilution, G-6, #sc-390189, Santa Cruz Biotechnology, Santa Cruz, CA, USA); other:Article Title: Redox state and altered pyruvate metabolism contribute to a dose-dependent metformin-induced lactate production of human myotubes Article Snippet: PDH-E1α , sc-377092 , |